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KMID : 0358920050320020357
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Journal of the Korean Academy of Pedodontics
2005 Volume.32 No. 2 p.357 ~ p.369
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IDENTIFICATION OF THE AG Ⅰ/Ⅱ AND GTFD GENES FROM STYEPTOCOCCUS MUTANS GS-5
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Jeong Jin-Woo
Kim Jae-Gon
Baik Byeong-Ju
Yang Yeon-Mi
Seo Jeong-Ah
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Abstract
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Streptococci are Gram-positive, facultative anaerobes and have no catalase activities. Among mutans streptococci containing a-type hemolytic activity, S. mutans is a causative agent for dental caries. As well as acid production yielding the demineralization of tooth enamel, adherence and colonization of S. mutans to the teeth are also important for its virulence. These early colonization are accomplished by the bacterial fibrillar protein, AntigenⅠ/Ⅱ (AgⅠ/Ⅱ), and glucosyl trans ferase (GTF). Therefore, Ag I / Ⅱ and GTF are reasonable targets for the development of vaccine against S. mutans GS-5. The agⅠ/Ⅱ and gtfD genes from S. mutans GS-5 were cloned and sequenced. Sequence analyses showed the nucleotides sequence of cloned genes had high homology to the sequences previously reported. The sequence alignment of 280 nucleotides between the cloned AgⅠ/Ⅱ and the available sequence of the corresponding S. mutans GS-5 showed the perfect match. Comparing with the sequence of gtfD from S. mutans UA159, the corresponding nucleotide sequence of S. mutans GS-5 showed some mismatches and the mismatches introduced changes in four residues out of 105 amino acids, yielding four missense mutations.
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KEYWORD
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Streptococcus mutans, Antigen Ⅰ/Ⅱ, Glucosyltransferase
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